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| Santos,Natália M.; Ferreira-Neto,Maressa; Artoni,Roberto F.; Vicari,Marcelo R.; Bakkali,Mohammed; Oliveira,Claudio de; Foresti,Fausto. |
| Individual samples from five populations of the characid fish Astyanax scabripinnis (Jenyns, 1842) from the Tietê and Paranapanema river basins (Brazil) were studied. All individuals analyzed presented 50 chromosomes but three different karyotypic forms were observed. Additionally, some individuals of one karyomorph presented a macro supernumerary chromosome. No chromosomal differentiation was observed between males and females in any sample analyzed. C-banding revealed two distinct distribution patterns in these populations in which strong terminally located heterochromatic blocks were detected in the long arms of some chromosomes of the specimens from Cascatinha, Cintra, and Funari streams, whereas populations of the Capão Bonito stream and the Barbosa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome markers; Karyoevolution; Karyomorphs; Nucleolus organizer region. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000200008 |
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| Teixeira,Thais K. S. S.; Venere,Paulo C.; Ferreira,Daniela C.; Mariotto,Sandra; Castro,Jonathan P.; Artoni,Roberto F.; Centofante,Liano. |
| ABSTRACT Astyanax is one of the most abundant and diverse taxa of fishes in the Neotropical region. In order to increase the amount of cytogenetic information for Astyanax as well as to exhibit data to subsidize future taxonomic studies, this work analyzed three species of Astyanax: two species are cryptic, and are here reported to live in syntopy (A. abramis and A. lacustris); the first karyotype description for A. pirapuan is also presented. Cytogenetic analyzes reveal a diploid number of 2n=50 chromosomes for three species, yet with differences in their karyotype morphology. The physical mapping of 18S rDNA showed up to thirteen sites in A. pirapuan and two in A. abramis and A. lacustris. The physical mapping of 5S rDNA has proven to be an effective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptic species; Fish; Karyotype; Species complex. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100214 |
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| Domingues,Manoela de S.; Vicari,Marcelo R.; Abilhoa,Vinícius; Wamser,João P.; Cestari,Marta M.; Bertollo,Luiz A. C.; Almeida,Mara C. de; Artoni,Roberto F.. |
| The recently described fish species, Astyanax altiparanae (tetra) is common in the upper rio Paraná basin, and has been reported in the Iguaçu basin. However, its natural origin in the rio Iguaçu is questionable. In the present work, karyotypical and morphological features of two populations of Astyanax altiparanae from the upper rio Tibagi and upper rio Iguaçu were compared. Both populations showed 2n=50 chromosomes and differences in their karyotype formula, NOR-bearing chromosomes and location of heterochromatin. Morphometric data from both populations were analyzed through free-size canonical variables. Cytogenetic and morphological results were mostly coincident showing exclusive markers that reflect their degree of populational isolation. In addition... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetra; Karyotype evolution; Distribution; Morphometry. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252007000100005 |
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| Castro,Jonathan P.; Moura,Maurício O.; Moreira-Filho,Orlando; Shibatta,Oscar A.; Santos,Mateus H.; Nogaroto,Viviane; Vicari,Marcelo R.; Almeida,Mara C. de; Artoni,Roberto F.. |
| Two populations of the Astyanax scabripinniscomplex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allopatric populations; Cytogenetics; Evolution; Geometric morphometry; Paraíba do Sul River. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000200429 |
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| Philippsen,Juliana S.; Renesto,Erasmo; Gealh,Ana Maria; Artoni,Roberto F.; Shibatta,Oscar A.; Zawadzki,Claudio H.. |
| Four samples of Neoplecostomus yapo were analyzed through the allozyme electrophoresis technique in corn starch gel. The allozyme pattern was similar to those found in N. paranensis with 24 loci scored. Two samples (ribeirão Atlântico and ribeirão Uraí) showed monomorphic bands for all 24 loci, whereas the other two (rio Verde and rio Fortaleza) showed 8.3% of polymorphic loci. The He genetic variability estimates for the rios Verde and Fortaleza populations were 0.0195 and 0.0179, respectively, too much inferior to the mean heterozygosity summed to species from the whole world (0.051). The Wright statistical values F IS = 0.5181, F IT = 0.5681 and F ST = 0.1039 and the genetic distance of Nei values showed that the four samples are genetically very... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Genetic variation; Neoplecostominae; Neotropical fishes; Siluriformes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000100004 |
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| Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto. |
| Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211 |
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| Bonatto,Naieli; Nogaroto,Viviane; Svidnicki,Paulo V.; Milléo,Fábio Q.; Grassiolli,Sabrina; Almeida,Mara C.; Vicari,Marcelo R.; Artoni,Roberto F.. |
| Maturity Onset Diabetes of the Young (MODY) presents monogenic inheritance and mutation factors which have already been identified in six different genes. Given the wide molecular variation present in the hepatocyte nuclear factor-1α gene (HNF1α) MODY3, the aimof this study was to amplify and sequence the coding regions of this gene in seven patients from the Campos Gerais region, Paraná State, Brazil, presenting clinical MODY3 features. Besides the synonymous variations, A15A, L17L, Q141Q, G288G and T515T, two missense mutations, I27L and A98V, were also detected. Clinical and laboratory data obtained from patients were compared with the molecular findings, including the I27L polymorphism that was revealed in some overweight/obese diabetic patients of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MODY3; Molecular diagnosis; Diabetes mellitus; Nucleotide sequencing. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500005 |
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